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Purpose
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This kit includes 1 mL of the 8 different MagSi-STA products and is intended for evaluation purposes during trial phase of developing new assays, or bead replacement in existing assays.
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Specificity
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Some IVD assays have requirements which are not covered by standard products in the market. The purpose of this kit is to find the best candidate for your assay application. However, if the products in this kit do not meet the requirements completely, we offer further customization on specific parameters. The initial evaluation of this MagSi-IVD trial kit will serve as a starting point for product optimization by our product development team. Our development of magnetic beads for IVD-use is focused on optimizing all relevant physical/chemical parameters. This customization approach will make your assay development more efficient, faster and cheaper.
Examples of product optimization:
Parameter: Product optimization options
Dynamic binding capacity (mean / variation): Coupling chemistry
Suspension time: Size / Magnetic content / storage buffer
Separation speed: Size / Magnetic content / storage buffer
Non-specific interaction: Adjusting functionalized surface / blocking agent
Optical density: Size
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Characteristics
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MagSi-STA beads are superparamagnetic spherical beads with a large surface made up by a nano-porous structure. The streptavidin coated beads are designed for any type of IVD sandwich assay (chemiluminescence-, fluorescence- or colorimetry-based) and can be applied on any IVD analyser.
Binding of molecules onto MagSi-STA is based on the strong non-covalent interaction between streptavidin and biotin (Ka = 1015 M-1). Biotin is easily conjugated to various molecules and inexpensive biotinylated products are sold by many companies. MagSi-STA beads are coated with recombinant streptavidin (53 kDa) with shortened N- and C-terminus for improved solubility and accessibility of the sites. Albumin s are eliminated for optimal specificity.
All MagSi beads included in this kit consist of a silica matrix, which does not have luminescent properties like for instance polystyrene. This results in comparatively low background signals in chemiluminescence- and UV/Vis read outs and enhances the assay sensitivity and dynamic range. Therefore, MagSi-STA beads are suitable for any assay, regardless of read out method used. The silica matrix ensures product stability over a wide pH range and at high ionic strength conditions as well.
Our production process has been developed for a very flexible but controlled magnetic bead synthesis and streptavidin coating. This process enables MagnaMedics to set the range for biotin binding capacity as narrow as needed (±10%). For some application this is important, because the parameters in play, like bead concentration, total amount of beads and binding kinetics of biotinylated targets, can be set as fixed values in optimizing and validating an IVD assay.
The streptavidin surface coating can be controlled with low variation for a broad range of desired binding capacities (up to 8000 pmol free biotin/mg beads, with ±10%) to ensure optimal reproducibility for your applications.
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Components
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This kit includes 1 ml of the 8 different MagSi-STA products
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Material not included
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Mixer/vortex to mix samples and re-suspend bead
Magnetic separator
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ProductDetails: Bead Ligand
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Streptavidin
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ProductDetails: Bead Matrix
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Magnetic Silica particles
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ProductDetails: Bead Size
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Bead size: 600 nm,1 μm,3 μm
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